羟基蛋氨酸锌对断奶仔猪镉损伤的修复作用

doi:10.11843/j.issn.0366-6964.2018.02.011

Abstract

Abstract:

This study aimed to investigate the protective effect of methionine hydroxy analog chelated zinc (MHZn) against cadmium inducing organ damage in weaned piglets. 24 barrows (Landrace×Large white, 45 day of age, (13.22±1.36)kg BW) were randomly allotted to 4 dietary groups (6 replicates per group, one piglet per replicate per pen):basal diet (control group, Con group), basal diet+30 mg·kg^-1 Cd (Cd group), basal diet + 30 mg·kg^-1 Cd+100 mg·kg^-1 MHZn (LMHZn group), basal diet+30 mg·kg^-1 Cd + 200 mg·kg^-1 MHZn (HMHZn group). The trial lasted for 30 days. On the morning of day 31, all of the piglets were weighted and blood samples were collected, then, all of the piglets were slaughtered and sampled. Liver, spleen and kidney were weighted to calculate organ coefficients. Average daily gain (ADG), average daily feed intake (ADFI) and the ratio of feed/gain (F/G) were calculated. We also conducted the histopathology examination of liver, kidney, duodenum, jejunum and ileum, the contents of Cd and metal trace elements in liver and kidney were detected, and the biochemical parameters in plasma were analyzed. The results showed that:1) Compared with Con group, piglets in Cd group had lower ADG and higher F/G ratio (P<0.05); dietary Cd also significantly decreased the villus length of duodenum and jejunum (P<0.05), increased crypt depth of duodenum and jejunum(P<0.05), decreased the ratio of V/C (P<0.05), increased Cd concentrations in liver and kidney (P<0.05). Hepatocellular degeneration and renal tubular degeneration were observed in piglets of Cd group. The concentrations of albumin in plasma significantly decreased after Cd treatment (P<0.05). 2) When piglets were fed diet with Cd and MHZn together, the ADG increased and F/G ratio decreased compared with piglets fed diet with Cd alone (P<0.05), and the value of ADG and F/D in LMHZn and HMHZn groups were not significantly different to Con group (P>0.05). Compared with Cd treatment, MHZn increased the villus length of duodenum and jejunum(P<0.05), suggesting that it could prevent the damage of intestinal villus. The liver coefficient in HMHZn group significantly decreased compared with Cd group (P<0.05), and the value of liver coefficient in HMHZn group was not significantly different to Con group (P>0.05), suggesting that MHZn relived hepatomegaly effectively. Besides, MHZn relived the granular degeneration and steatosis in liver, reduced renal tubular degeneration and lymphocyte proliferation in kidney. Moreover, MHZn (200 mg·kg^-1) decreased Cd accumulation in liver and kidney compared with Cd group(P<0.05), reduced the impact of Cd on albumin and total protein in plasma, suggesting that MHZn was helpful in reliving the Cd inducing damage on liver, kidney and gut. In summary, MHZn has beneficial effect on reliving and repairing the damage of small intestine, liver and kidney caused by Cd.

CLC Number:

S828.5

LIU Fen-fen, NI Heng-jia, HUANG Pan, WU Xin, ZHANG Bin, ZHANG Wei-jun, YAO Ya-jun, YIN Yu-long. The Protective Effect of Methionine Hydroxy Analog Chelated Zinc on Cadmium Inducing Damage in Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 318-326.

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The Protective Effect of Methionine Hydroxy Analog Chelated Zinc on Cadmium Inducing Damage in Weaned Piglets

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